Composite
Part:BBa_K567001:Design
Designed by: Chunying Li and Yunfeng Ruan Group: iGEM11_SJTU-BioX-Shanghai (2011-09-29)
lacI-Ptrc-tRNA(Arg)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1845
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1845
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1845
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1845
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2026
Design Notes
The correct processing to produce mature tRNA should be ensured.
Source
LacI- Ptrc derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.